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Full-length 16S rRNA gene sequencing is carried out using a significantly better form of our very own way for artificial much time-understand sequencing19. To improve amplification from bacterial 16S rRNA gene variety for the long-understand sequencing, i changed the fresh 1492r primer before used18 to your 1391r primer48 because it has better coverage of the known bacterial diversity. At the same time, we changed the new Taq polymerase used in number 1 amplification of your novel molecular identifier (UMI) tagged themes having a great proofreading polymerase (Additional Fig. 16). It enhanced the new portion of mistake-free reads nearly five-bend, and this significantly advances all of our capacity to look after complete-size 16S rRNA ASVs (FL-ASVs) of lower plentiful taxa since the no less than a couple similar sequences is needed to look after a keen Florida-ASV18.
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